渔船型线计算机辅助设计模型及软件系统的研究

本文重点讨论了在计算机辅助渔船型线设计中采用的数学模型，应用数据库技术生成和逐渐完善母型船的型船库系统，以及采用三次样条函数插值解决光顺问题和软件系统。     

利用RAP-PCR检测高盐刺激下Shewanella piezotolerans WP3的基因差异表达

选取高盐刺激为条件，利用实验室模拟极端环境培养条件下的菌体提取RNA，采用差异显示方法RAP—PCR扫描盐刺激下基因的差异表达。结果表明，在高盐刺激下，WP3下调表达的基因是SSl：hypotheticalproteins SS2：ferroxidase。上调的基因是SS3：gamme-butyrobetaineantiporter；SS4：hypothetical protein。     

Selective one-step extraction of Arabidopsis thaliana seed oleosins using organic solvents

Oleosins are hydrophobic proteins from oleaginous seeds, surrounding and stabilizing oil bodies. They are known to display interesting interfacial properties. Specific sera were raised against four different A. thaliana oleosins and used in dot-blot assays for oleosin quantification. These assays were used to set up extraction of oleosins from A. thaliana seeds. One mixture of chloroform/methanol gave optimal oleosin extraction. Extracted proteins represented 9% of seed proteins and were identified by immunoblot and proteomic analyses. Oleosins accounted for 79% of the extracted proteins. This simple one-step procedure allows selective extraction and concentration of oleosins from seeds without tedious oil body purification. Oleosin extract was indeed used to demonstrate the presence of the rare oleosin S5 in mature seeds. Moreover, this method will be useful to investigate the potential use of oleosins as emulsifier and to question their possible allergenicity.     

海洋菌W11产中温淀粉酶的酶学特性

本研究从威海文登海域筛选获得一株产淀粉酶海洋菌W11,初步鉴定为弧菌,并探讨pH、温度、无机离子对淀粉酶活性和稳定性的影响及该酶底物浓度效应和Km值。结果表明：在pH7.5左右酶活性最高,pH在4.0～7.5范围内体现较强的稳定性。最适酶解温度为55℃,酶液在60℃以下有较好的热稳定性;Ba2＋、Mn2＋对淀粉酶有激活作用,而Cu2＋、Mg2＋、Zn2＋则抑制淀粉酶活性,表观Km值为0.973mg/mL。海洋菌W11所产的中温淀粉酶保存温度范围较广、适应pH作用的范围广及稳定性较强,将有着广泛的应用潜力。     

疏浚物倾倒对岚山港临时海洋倾倒区周边海域环境影响的研究

为了解疏浚物倾倒对岚山港临时海洋倾倒区周边海域生态环境造成的影响,采用单因子质量指数法和综合指数法对该倾倒区周边海域海水和沉积物历年跟踪监测结果进行评价,并对该倾倒区周边海域海洋生物以及水深的变化情况进行分析。结果表明:该倾倒区周边调查海域历年水质质量符合《海水水质标准》(GB 3097—1997)中第二类水质标准,除无机氮外,其他各评价因子的质量指数较为稳定,基本在一定的范围内波动;沉积物质量符合《海洋沉积物质量》(GB 18668—2002)中第一类海洋沉积物质量标准,沉积物各评价因子质量指数均在小范围内波动;疏浚物的倾倒对该倾倒区海域浮游植物种类数和密度造成暂时性的减少;疏浚物的倾倒造成倾倒区东部和北部海底各形成一个明显的凸起地形。可见除倾倒区的水深受到影响外,疏浚物的倾倒尚未对该倾倒区海域生态环境质量造成明显影响。     

Study on the Quantity Dynamic Changes of Heterobacteria and Vibrios in Larvae Industrialized Culture System

[Objective] The dynamic change of heterobacteria and vibrios in larvae industrialized culture system was studied to provide scientific reference for healthy cultivation of shrimp.[Method] The heterobacteria,vibrios and pathogenic vibrio parahaemolyticus were monitored in larvae industrialized culture system.[Result] The heterobacteria,vibrios and pathogenic vibrio parahaemolyticus were the most in fertilized eggs of shrimp but the least in nauplius,then their number would increase with growth. During whole rearing period,both heterobacteria in larvae,vibrios in water would increase by one order of magnitude,while both vibrios in larvae and heterobacteria in water would increase by two orders of magnitude.There were many heterobacteria and vibrios but few vibrio parahaemolyticus in living bait.The correlation coefficients between larvae and heterobacteria and vibrios in water were 0.704 and 0.840 in culture system respectively,while the correlation among heterobacteria,vibrios in living bait and larvae,water were weak or negative.[Conclusion] There was a dynamic relation between water and larvae in rearing period,and restrictly control of culture condition would restrain the occurrence of disease caused by vibrio parahaemolyticus,besides that bacteria number in bait was not obviously correlated with bacteria nubmer in culture system.     

桁拖网渔具刚性栅栏对虾类的分隔性能

刚性栅栏是实现拖网渔具种类选择性捕捞的主要装置。通过海上生产试验,结合SELECT模型分析不同栅条间距（15mm、20mm和25mm）的刚性栅栏对哈氏仿对虾（ Parapenaeopsis hardwickii ）和葛氏长臂虾（ Palaemon graxieri ）的分隔性能：试验结果显示,随着栅条间距的增大,栅栏对虾类的重量分隔率逐渐增大;个体接触分隔栅栏的慨率呵使用常数来表示,即接触慨率与个体尺寸无关;比较不同虾类对分隔栅栏的接触慨率后发现,接触慨率不存在种类间、栅栏间的显著性差异（P〉0．05）;个体接触分隔栅栏的概率均大于0．9,说明大多数个体都接触到分隔栅栏,但拒绝全部接触栅栏的假设,这主要与桁拖网结构和分隔栅栏的安装有关;随着栅条间距的增大,栅栏对虾类的50％选择体长（L50）逐渐增大,但选择范围（SR）没有显著差异。     

饲料中不同磷水平对红螯螯虾幼虾生长和体组成的影响

以秘鲁鱼粉、豆粕为蛋白源,鱼油和豆油为脂肪源,通过调节饲料中不同磷酸二氢钙水平形成7种不同磷水平(0.72%、0.85%、1.00%、1.16%、1.32%、1.47%和1.74%)的等氮等能饲料,并以此饲料投喂红螯螯虾幼虾(2.25±0.12)g8周。试验结果显示:饲料中不同磷水平对红螯螯虾幼虾的增重率、特定生长率、饲料系数和成活率都有一定的影响,增重率和特定生长率均随着磷水平的升高而升高,当总磷含量为1.34%时获得最佳特定生长率,而过高(1.74%)或过低(0.72%)的磷水平会显著影响幼虾的生长;饲料系数随磷水平的升高呈下降趋势;在1.00%～1.74%的磷水平之间成活率均为100%;随着磷水平的升高,全虾和肝胰脏粗脂肪含量逐渐降低,全虾粗灰分和总磷含量均逐渐升高,并且肌肉粗蛋白含量也呈一定的增长趋势;适宜的磷水平可以提高血浆碱性磷酸酶活性,而肝胰脏碱性磷酸酶活性随磷水平的升高而降低。综合幼虾的生长性能、营养成分等分析得出,体重在(2.25±0.12)g的红螯螯虾幼虾适宜的饲料总磷含量在1.32%～1.47%之间。     

智利外海茎柔鱼繁殖生物学初步研究

对2007年-2009年我国鱿钓船在东南太平洋智利外海生产期间所采集的1 374个茎柔鱼样品性成熟度进行观察,初步研究了其繁殖生物学特性。结果表明:智利外海茎柔鱼为大型群体,常年产卵,无明显的产卵高峰期。渔获物雌雄比例约为2.65∶1。雌、雄个体性腺成熟度差异不明显,其中成熟个体所占比例小,分别占总量的3.9%和6.7%。缠卵腺长和缠卵腺重随着性腺成熟度增加逐步增大,成熟个体性腺指数明显大于未成熟个体。雌、雄个体性腺指数存在差异,雌性个体Ⅳ期性腺指数与胴长成正比,而雄性则成反比。雌性和雄性茎柔鱼的初次性成熟胴长(ML50%)分别为638.3 mm和565.3 mm。